Friday, May 9, 2008

Fitzpatrick’s Bio Skin Device




Bioskin device is a generator of UV-B radiation composed of three
main components:

1. UV-B short arc mercury lamp (100 Watt) which generates UV-B
with a spectrum of 280 to 320 nm with a maximum emission peak
at 311 nm;
2. Specialized liquid component optical fiber which can transmit
and focalize the emitted UV-B in a circular beam 1 cm in diameter.
3. Computerized system which allows the regulation of the
intensity (10-100 mJ/cm^2 /s) and time of single spot emission
(0.1-10 seconds).

/Light spot./

Each single spot produces an energy of 10-100 mJ/cm^2 on a 1 cm
diameter circular area (0.785 cm^2 ) for the time necessary to reach
the optimal dose. The optical fiber terminal is in contact with a
different site of the treated patch during the emission of each
spot. Repetition of single spots make it possible to treat VP areas
completely while avoiding normal skin. Complete treatment of a 10
cm^2 diameter vitiligous area with 100 mJ requires repeating a 2
second 1 cm^2 diameter spot 6 times with 100 mJ/cm^2 intensity.

/Treatment session/

Each treatment session consists of irradiation of the VP with a dose
20% lower than minimal erythema dose (MED) calculated by the
operator before the session. The length of each session depends on
the length of the single light spot and the extension of the VP areas.

/Treatment./

The MED in VP is evaluated 24 hours before the beginning of therapy.
The subjects are treated with the following scheme:

* 5 sessions, one a day for 5 consecutive days.
* 10 days break.
* 1 session every 15 days for 5 months.

The control subjects were treated with the same protocol but with
the UVB generator off. It was impossible for the control subjects to
know if the generator was on or not.

Table 3 shows the MED for each patient, dose per session for cm^2 ,
total dose given each subject per cm^2 and the final results.

Vitiligo Microphototherapy


Vitiligo is a common disease of unknown cause that
produces disfiguring white patches of depigmentation. Previous
studies have suggested the effectiveness of UV-B radiation in
generalized vitiligo (GV) therapy, but there was no evidence to
support the same role for segmental vitiligo (SV).

*Objective:* The purpose of this study was to use UV-B radiation
exclusively on vitiligo patches of individuals affected by SV to
evaluate the effectiveness of this therapy.

*Subjects & Methods:* 8 individuals with SV were treated for six
months with a new device called BIOSKIN ® that can produce a focused
beam of UV-B (microphoto-therapy) on vitiligo patches only.
Photographs of the subjects were taken at the beginning of the
therapy and once a month thereafter for six months. The response to
treatment was estimated in 2 comparable photographs using
planimetry. A control group of 8 individuals matched for sex and age
was treated with placebo, using the same device but not releasing
any kind of detectable light.

*Results:* After six months of microphototherapy 5 subjects of the 8
studied achieved normal pigmentation on more than 75% of the treated
areas. In particular, 3 of these were totally repigmented. Two
individuals achieved 50-75% pigmentation of the treated areas, and
only one showed less than 50% repigmentation (table 3). In the
control group only one patient showed moderate repigmentation (less
than 50%) (table 3) (Figure 1).

*Conclusion:* UV-B microphototherapy seems highly effective in
restoring pigmentation in patients affected by vitiligo. As no side
effects have been observed, this could represent the treatment of
choice in the limited (segmental) forms of vitiligo.

*Keywords:* vitiligo, UV-B, therapy



*Introduction
*
Vitiligo is an acquired hypomelanotic disease of unknown etiology
affecting 1-2% of world population without any racial, geographic or
sex differences (1). Although use of ultraviolet-B (UV-B) radiation
in vitiligo therapy is relatively recent, it is considered presently
the most effective treatment for generalized vitiligo (1,2).

The successful use of UV-B rays is probably due to several direct
and mediated interactions of UV-B with melanocytes, keratinocytes
and skin immune system (Table 1).

Enhancement of pigmentation
- By increase of melanocyte stimulating hormone (MSH) receptor
binding activity and melanocortin receptor gene expression [3]
- By activation of cyclic-AMP pathway by alpha-melanotropin which
increases melanocyte proliferation and melanogenesis [4]
- By irradiated keratinocyte production of nitric oxide (NO)
(paracrine induction of melanogenesis) [5]
- By increase of tyrosinase mRNA expression and enzymatic activity [6]
- By melanocyte production and secretion of corticotropin releasing
factors [7]
lnduction of skin inflammation
- By enhancement of keratinocyte production and release of TGFß-1 [8]
- By enhancement of keratinocyte production and release of IL-1 [9]
Alteration of local (skin) immune system response
- By enhancing production and release of TGFß-1which causes
immunosuppression [8]
- By enhancing release of cis urocanic acid (cis-UCA) [10]
By enhancing keratinocyte production and release of TNFß [11]
Tumor promotion
- By induction of c-jun and c-fos protooncogene transcription in
keratinocytes [12]
- By causing cellular DNA damage
Cellular programmed self destruction
- By increasing keratinocyte levels of tumor suppressor gene p53 [13,14]
- By increasing keratinocyte Ievels of 1.25 dihydroxyvitamin D3,
TGFß-1,Ca ^2+ [15]
Metabolic alteration
- Enhanced production of free radical levels
- Enhanced superoxid dismutase (SOD) levels and activity [16]

Table 1 - The main direct and mediated effects of UV-B irradiation
of the skin

In this study we used a new device called BIOSKIN^® provided with a
focused beam of UV-B adapted to treat selected areas of depigmented
skin.

*Subjects and Methods*

/Subjects/

Subjects with segmental vitiligo were included in the study after
obtaining informed consent to ensure that the procedure of
microphototherapy had been fully explained. The individuals were 4
men and 4 women with a mean age of 17.9 years and skin type III for
6 persons and II for the other 2. The control group was composed of
8 individuals, 5 men and 3 women, affected by SV with a mean age of
22.9 years; skin type was III for 5 persons and II for the other 3.

Table 2 shows the sex, age, Fitzpatrick skin phototype and affected
areas for each subject treated.

Ultraviolet Sensor

This sensor measures the ultraviolet radiation between 250 and
400 namometers in μmol m-2 s-1 (micromoles of photons per sqare
meter second).
Although the relative wavelengths of UV radiation differ among
sunlight and electric lights, our measurements, shown in the graph
below, indicate that this sensor provides a close estimate of the UV
radiation coming from electric lamps. This sensor is particularly useful
for determining the UV filtering capacity of the transparent plastic and
glass barriers that are commonly used below electric lamps.

Attach the sensor to a meter or datalogger
capable of displaying or recording a mV output.
The model, serial number, production date, and
conversion factor are located on the sensor cable.

Mount the sensor as level as possible. Small
changes in level can cause measurement errors. We
recommend using our leveling plate (model LEV) for
the most accurate measurements.
The sensor should be mounted with the cable pointing
toward the nearest magnetic pole to minimize azimuth
error.

Why this sensor cannot selectively
measure UV-B Radiation (280-320 nm)
Our measurements confirm those of others and indicate that less than
0.4 % of the photon flux from sunlight falls below 320 nm; 2.3 % falls
between 320 and 350 nm, and 6 % falls between 350 and 400 nm. Although
the UV radiation between 250 and 320 nm is critically important
in photochemical and photobiological reactions, only about 5 % of the UV
photons are in this range. Because only a small fraction of the photons
are in the UV-B range, this meter cannot be used to selectively measure
UV-B radiation. The sensor is sensitive to UV-B radiation, but it is included
with the UV-A radiation to provide a total measurement of UV radiation.

Effects on Output
Level
The sensor must be exactly horizontal for the most
accurate measurement. The largest error is often
caused by small changes in the position of the sensor.
The sensor should be mounted with the cable pointing
toward the nearest magnetic pole.
Cosine response
Some of the radiation coming into a sensor at low
angles is reflected, which causes the reading to be less
than it should be. The cosine-corrected head helps to
capture radiation at low angles. The cosine error for
typical applications is less than 10 %.
Temperature response
The temperature response is about 0.1 % per degree
celsius. This temperature error is insignificant for most
applications.
Long-term stability
The output of all radiation sensors tends to decrease
over time as the detector ages. Our measurements
indicate that the average decrease of the sensor is
about 1 % per year. We recommend returning the
sensor for recalibration every 3 years.

Specifications
435-792-4700
www.apogeeinstruments.com
techsupport@apogee-inst.com
Absolute accuracy ± 10 %
Uniformity ± 5 %
Repeatability ± 1 %
Output Responsivity Approximately 0.15 mV per μmol m-2 s-1
In full sunlight Approximately 26 mV (170 μmol m-2 s-1)
Linear range 0 to 400 μmol m-2 s-1
Sensitivity Calibrated to approximately 6.5 μmol m-2 s-1 per mV
Input power None, self-powered
Operating environment Can be submerged underwater (with or without
mounting bolt).
Materials PVC head, potted solid
Cable 3 meters of shielded, twisted-pair wire with
Santoprene casing, ending in pigtail leads.
Additional cable $1.95/meter.
Dimensions 2.4 cm diameter, 2.75 cm high
Mass 70 g (with 3 m lead wire)
Warranty 1 year parts and labor

Handheld Readings
1. Turn the dial clockwise to the “on” position.
2. Handheld UV meters should be held level as shown
below. Separate sensors should be mounted on a
horizontal surface.
3. The number displayed is the μmol m-2 s-1
4. Turn the meter off after use to conserve battery
power.

Calibration
Although the relative wavelengths of UV radiation differ
among sunlight and electric lights, our measurements, shown
in the graph below, indicate that this sensor provides a close
estimate of the UV radiation coming from electric lamps. This
sensor is particularly useful for determining the UV filtering
capacity of the transparent plastic and glass barriers that are
commonly used below electric lamps.

Why this Meter cannot selectively
measure UV-B Radiation (280-320 nm)

Our measurements confirm those of others and indicate that less than
0.4 % of the photon flux from sunlight falls below 320 nm; 2.3 % falls
between 320 and 350 nm, and 6 % falls between 350 and 400 nm. Although
the UV radiation between 250 and 320 nm is critically important
in photochemical and photobiological reactions, only about 5 % of the UV
photons are in this range. Because only a small fraction of the photons
are in the UV-B range, this meter cannot be used to selectively measure
UV-B radiation. The sensor is sensitive to UV-B radiation, but it is included
with the UV-A radiation to provide a total measurement of UV radiation

Sources of Error in UV Radiation

The variety of applications of ultraviolet (UV) light and the consequent need for accurate UV measurements
have increased enormously over the last 20 years. In some cases, the UV radiation from a source is of inter
(e.g., tanning booths and solar radiation). At other times, the action or chemical reaction initiated by UV
irradiation of a system is of interest (e.g., water purification,UV curing, and semiconductor photolithography).
Finally, UV radiation has a cumulative deleterious effect on biological systems; there are consequently health
safety requirements for the accurate measurement of UV radiation.

Considerable effort has been made to produce simple instrumentation to meet these wide-ranging UV
measurement needs. The typical UV meter or radiometer is composed of a number of simple optical elements,
as shown in Fig. 1. The incident radiation passes through an aperture that limits the active area of the system.
diffuser is often placed after the aperture and is used to improve the angular response and spatial uniformity
The instrument. An optical filter is then employed to select the spectral region of the incident optical
radiation that strikes the detector.

To fully understand the accuracy of such a UV meter, the optical properties of its components and the spectral
responsivity should be known as well as the relative spectral distribution of the source. Additionally, the UV
meter will seldom perform ideally, and out-of-band, non-linear, and non-ideal geometric or spatial response
must be characterized to achieve the lowest uncertainties. However, most UV meters are supplied from the
manufacturer with a calibration at a specific wavelength, and only a nominal wavelength band is specified. In
addition, the spectral distribution of the source being measured is often unknown. The purpose of this paper
is to illustrate that considerable thought must be given to the utilization and calibration of these simple
devices order to understand and minimize measurement errors

2. Sources of Error

It is important to define, at the outset, the physical quantity that is to be measured and the level of uncertainty
needed to achieve the measurement goals. The measurement requirements for the UV meter can be
very different: spectrally integrated irradiance (W/cm2) in the UV-A (315 nm to 400 nm) or UV-B (280 nm to
315 nm) regions as in the case of solar irradiation; a single wavelength dose or exposure (J/cm2) as in the
case of semiconductor photolithography; or an effective or weighted dose (Effective J/cm2) as in the case of
biological action spectra. The sources of error in optical radiation measurements described here are not new
to radiometry.These errors in addition to measurement techniques and procedures are well documented in the field of
photometry. However, these topics are less well known in the UV radiation measurement community,
especially among novice users of UV measurement instruments.Due in part to increasing UV applications, recent
publications specifically address UV meter calibration and characterization [2, 3]. In the following, we discuss
common sources of error in UV radiation measurements,including out-of-band contributions to the signal,
non-ideal geometric properties (non-ideal cosine response in the meters), and poor matching to a defined
action spectrum. Other sources of error have been discussed in the literature and will not be discussed here.
These include environmental factors such as temperature and humidity,which can lead to wavelength-dependent
responsivity changes in UV meters. In addition, UV radiation itself induces aging of the optical elements of meters.
Finally, optical detectors used in UV meters have a finite range over which they have an output signal linearly
proportional to the incident irradiance. UV meters should be tested to verify that they are in the linear range both
for the irradiance level used in practice as well as for the smaller levels typically used for calibration.

3. Out-of-Band/Non-Ideal Responsivity

An ideal meter would have a well-defined responsivity within a specific spectral region and zero responsivity
outside of this region. For example, an ideal UV-A meter would have a constant responsivity from 315 nm
to 400 nm and no response outside of this region.Figure 3 shows the spectral responsivity, determined
in monochromatic radiation, of two broadband UV meters used in semiconductor photolithography to
determine the total exposure of a photoresist to 365 nm radiation from a filtered mercury source [4]. These
meters have a maximum responsivity in the 365 nm region, and the responsivity then decreases to a much
smaller, though non-zero, value at longer wavelengths. The instruments demonstrate differing amounts of
increased responsivity in the near infrared (IR), with Meter A showing responsivity 2 to 3 orders of magnitude
larger than Meter B in the 700 nm to 1000 nm spectral region. The increased IR responsivity is due to
increased transmission in the IR by the glass filters, and because silicon photodiodes have their peak response
in the near IR. The increased responsivity observed at wavelengths shorter than 300 nm is caused by fluorescence
of the diffuser, which then re-emits longer wavelength radiation that passes through the filter to the
photodiode. This was verified in Meter A by placing the diffuser between the filter and the photodiode. This
effectively eliminated the responsivity near 275 nm.For monochromatic radiation measurements near
365 nm, the out-of-band response is not important and both meters can make measurements with little error.
Many real optical sources that are assumed monochromatic,such as lasers, often emit radiation
at additional wavelengths. If the source to be measured emits flux at wavelengths below 300 nm or above
680 nm, the 365 nm radiation could be overestimated and measurements with these two meters will
disagree. Although these UV meters were designed to measure monochromatic radiation, they are very similar to UV
meters designed and used for broadband UV radiation.To illustrate these errors, we compare the signal
produced by the two UV meters from four typical sources with different spectral power distributions: a
mercury arc lamp, a quartz-tungsten halogen lamp (ANSI designation, FEL), a deuterium lamp, and a
xenon arc lamp. The relative spectral distribution of each source is shown in Fig. 4.
Using Eq. (1), we compare the integrated in-band irradiance signal with the out-of-band signal. The
in-band signal is the product of the spectral distribution of the source and the meter responsivity, integrated over
the spectral region from 315 nm to 400 nm. The out-ofband response is the integral of the product summed
over the 200 nm to 315 nm and 400 nm to 1000 nm spectral ranges.

OZONE AND SOLAR UV-B ERYTHEMAL IRRADIANCE

ABSTRACT
Results of the total column ozone and ultraviolet (UV-B) erythemally weighted irradiance
measurements at the ground-based solar monitoring station at the Kishinev (Moldova) are
presented. Diffuse and global components of solar UV-B erythemal irradiance on horizontal
plane were continuously measured with sensors UV-S-B-C (of broadband 280-315 nm),
Kipp&Zonen. Monthly totals of global and diffuse components of solar UV-B erythemal
radiation reveal distinct seasonal variation with respective minimum in winter and maximum in
summer. Typical values for these components in limiting cases are presented. A simple
polynomial relationship between the global and diffuse components of solar UV-B erythemal
radiation measured for cloudless days was derived. It was shown that coefficients of the
polynomial depend on daily mean value of aerosol optical thickness (AOT). Collocated
measurements of AOT have been carried out with the sunphotometer Cimel CE-318 within
the framework of the Aerosol Robotic Network (AERONET) program, managed by
NASA/GSFC.
Total column ozone content was retrieved from direct solar ultraviolet radiation measurements
at 3 discrete wavelengths centered at 305.5, 312.5, and 320 nm within the UV-B range.
Ozone measurements were regularly carried out with the hand-held MICROTOPS II
Ozonemeter, Solar Light Co. Monthly average values of total column ozone content
measured with the MICROTOPS II at the Kishinev are in close agreement with those ones
retrieved from the multiyear (1978-2004) database statistics acquired from satellite platforms
measurements with the Total Ozone Mapping Spectrometer (TOMS). It was shown the
existence of seasonal variability of the total column ozone content with respective minimum
values observed at the end of autumn and winter, and maximum values observed at the end
of winter and in spring. The maximum and minimum of daily mean values of total column
ozone ever measured with TOMS at the satellite platforms overpassed Kishinev site,
amounted of ~540 DU (on February 19, 1985) and ~204 DU (on December 1, 1999). Yearly
mean value of total column ozone measured at the Kishinev was ~ 338 DU. Total column
ozone measurements carried out with MICROTOPS at the Kishinev site from September
2003 to August 2004, gave maximum and minimum values of ozone daily means at ~ 489 DU
(on February 12, 2004) and ~259 DU (on December 3, 2003). The estimation of total column
ozone trend derived from the TOMS multi-year statistics was ~ -10 DU/decade.
KEYWORDS: column ozone content, UV-B erythemal radiation, aerosol optical thickness.
1. INTRODUCTION
Ozone and aerosol particles in atmosphere modify the intensity and spectral composition of
the solar ultraviolet radiation at the Earth’s surface. Each of components has a specific
influence upon the radiation exchange and interaction processes that finally define of solar
UV-B radiation reaching the surface. Aerosols produced by both human activities and natural
processes affect the UV-B radiation within the whole atmosphere through the scattering and
TOTAL COLUMN OZONE AND SOLAR UN-B ERYTHEMAL IRRADIANCE 205
absorption processes. The absorption of the UV-B radiation is largely controlled by ozone in
the stratosphere at altitudes between 25 and 100 km. There are, however, other effects that
influence the UV-B radiation transfer: cloud cover, tropospheric ozone, other gaseous
pollutions, surface albedo. To investigate complex relationships between various phenomena
taking place in the atmosphere it is necessary to have reliable total column ozone and solar
UV-B radiation data obtained from the ground-based measurements. As a consequence of
ozone and aerosols can change the UV-B exposure both locally and globally, ground-based
solar radiation monitoring stations become of a particular of interest to obtain an exhaustive
and reliable continuous flow of information about the resulting UV-B erythemal radiation field
on the Earth’s surface and the total column ozone content at the sites of observation.
This paper discusses results of the total column ozone and solar broadband ultraviolet (UV-B)
erythemal weighted irradiance measurements carried out at the ground-based solar
monitoring station at the Kishinev, Moldova.
2. MEASUREMENT APPROACH
For the first time in Moldova it was established ground-based station for continuous solar
radiation monitoring. Station was equipped with the solar radiation sensors for broadband
measurements of radiation from UV-B to IR, data logger CR10X, and active solar tracker unit
2AP BD, (Kipp&Zonen). These instruments were assembled into the multifunctional
radiometric complex. Additional instruments, such as an automatic weather station MiniMet,
ozonemeter MICROTOPS II and sunphotometer Cimel CE-318, are used at the station. Data
sets collected from weather station MiniMet (Sky Instruments Ltd.), which is arranged at a
distance of 30 meters apart from the radiometric measuring complex, supplement the solar
radiation measurements. Radiometric complex is placed in an urban environment at the
Kishinev site (see Fig. 1) with coordinates: ϕ=47.00130N, λo=28.81560E, h=205 m a.s.l. All
instrumentation was mounted on the roof of the building of the Institute of Applied Physics,
Academy of Sciences of Moldova.
Radiometric complex is used to carry out long-term continuous monitoring of solar radiation at
the Earth’s surface. Measurements are made with 1 sec resolution and 1 minute averaging
interval. Solar radiation sensors used at the station allows for covering wavelength range from
UV-B to IR and to make broadband measurements of global, diffuse and direct components
of solar radiation. Diffuse and global components of the solar UV-B irradiance are measured
with two sensors UV-S-B-C (of broadband 280-315 nm) installed at the moving platform of the
active solar tracker 2AP BD unit and mounted at the stationary platform, respectively. Solar
UV-B erythemal weighted irradiance is re-calculated by using the specific adjustment tables
for each sensors taking into account the solar zenith angle and total column ozone content at
the site of observation.
Typical values of measured global and diffuse components of monthly totals of solar UV-B
erythemal radiation are presented. Total column ozone content is regularly measured at the
station by hand-held narrowband filter MICROTOPS II Ozonemeter, (Solar Light Co) [1]. This
instrument is equipped with the highest grade and long stability filters with ion-beam assisted
deposition and centered at λ= 305.5, 312.5 320, 936 & 1020 nm. MICROTOPS II Ozonemeter
gives an accuracy < mquvb ="8.5" mduvb ="7.4" mquvb="144.5" mduvb =" 104.7" y=" C"> measured with the sunphotometer Cimel CE-318 at
λ=500 nm (see Figure 4). Coefficient A has a little dependence on <τa(500)> and mean value
of
is ~ 1.1, whereas coefficient B has strong dependence on <τa(500)>. Coefficient B
decreases with the increase of AOT <τa(500)> . For the set of clear free days these
scattergrams have the analogous dependence, but with specific A and B coefficients. For
overcast days coefficient A tends to be equal to the ratio QUVB/ DUVB ~1.07 and coefficient B
becomes equal to 0.0.
Figure 3. Scattergram of minute average
values of global vs. diffuse components of the
solar UV-B erythemal irradiances for cloud free
day on September 6, 2004.
Figure 4. Variation of coefficients A and
B versus daily means of aerosol optical
thickness <τa(500)> at λ=500 nm for
selected cloud free days from April to
September 2004.
Figure 5 shows monthly average values of total column ozone content retrieved from
multiyear (1978-2004) statistics retrieved from TOMS measurements and measured with
hand-held MICROTOPS II ozonemeter at the Kishinev site during September 2003 – August
2004. It is clear seen the existence of seasonal variability of total column ozone content with
minimum observed at the end of autumn and in winter, and maximum observed at the end of
winter and in spring. Ozone values obtained from measurements made with MICROTOPS
208 ACULININ
and retrieved from TOMS measurements are in good agreement with each other. The most
scatter in the data measured with MICROTOPS was observed from December to March. The
error bars show one standard deviation. Figure 6 shows multiyear (1978-2004) statistics of
the yearly average values of total column ozone content retrieved for the Kishinev site from
the measurements made at the satellite platforms with the TOMS.
Figure 5. Monthly average values of total
column ozone content retrieved from
multiyear (1978-2004) statistics from TOMS
measurements and measured with hand-held
MICROTOPS II at the Kishinev site during
September 2003 – August 2004.
Figure 6. Multiyear (1978-2004)
statistics of the yearly average values
of total column ozone content retrieved
from measurements made with TOMS
at the satellite platforms overpassed
Kishinev site.
The maximum and minimum of daily mean values of total column ozone ever measured with
TOMS from satellite platform overpassed Kishinev site, amounted of ~540 DU (on February
19, 1985) and ~204 DU (on December 1, 1999), respectively. The last extreme value of
column ozone content is attributed to the mini ozone holes evolution over the West and
Central Europe in December 1999. The estimation of total column ozone trend gives the
value of ~ -10 DU/decade. Total column ozone measurements carried out with MICROTOPS
at the Kishinev site from September 2003 to August 2004, gave maximum and minimum
values of ozone daily means at ~ 489 DU (on February 12, 2004) and ~259 DU (on
December 3, 2003), respectively. Yearly mean values of the total column ozone content
derived from ground-based measurements at the Kishinev site and retrieved from the multiyear
statistics of the TOMS measurements were very close to each other and gave the
values of ~ 338 DU and ~334 DU, respectively.
4. CONCLUSIONS
Continuous solar radiation measurements from UV-B to IR have been carrying out at the solar
radiation monitoring station established in an urban environment of Kishinev. Period of
observation was chosen from October 2003 to September 2004. Results of measurements of
the monthly totals of global and diffuse components of solar UV-B erythemal radiation (with
UV-S-B-C sensors of broadband 280-315 nm) on horizontal plane are presented. It was
shown seasonal variation of these components with the presence of minimum (for winter
season) and maximum (for summer) of their values. It was shown the influence of the number
of overcast days upon the variation of the monthly totals of sunshine duration. The regression
relationship of the scattergram for global QUVB and diffuse DUVB components of solar UV-B
erythemal radiation measured for cloud free days may be represented by second order
polynomial regression curve. The regression coefficient at term of order two has strong
dependence on aerosol optical thickness <τa(500)> measured with the sunphotometer Cimel
CE-318 at λ=500 nm: this coefficient decreases with increasing of the aerosol optical
thickness. Coefficient at term of order one is practically independent on <τa(500)>, and free
term is negligible one.
TOTAL COLUMN OZONE AND SOLAR UN-B ERYTHEMAL IRRADIANCE 209
Total column ozone trend gives the value of ~ -10 DU/decade. This value was derived from
the multiyear (1978-2004) statistics of the TOMS measurements. Measurements of total
column ozone made with MICROTOPS at the Kishinev site from September 2003 to August
2004, gave maximum and minimum values of daily means of ozone with ~489 DU (on
February 12, 2004) and ~259 DU (on December 3, 2003), respectively. Yearly mean value of
total column ozone measured with ozonometer at the Kishinev site was ~ 338 DU.
ACKNOWLEDGEMENTS
I thank Dr. Brent Holben, the Principal Investigator of the AERONET program (NASA/GSFC) and
his staff in supplying sunphotometer Cimel-318 used in this investigation at the Kishinev site and
data processing. The work was funded by the U.S. Civilian Research &Development Foundation
(CRDF) and the Moldovan Research and Development Association (MRDA) through grant #ME2-
3033.
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1. Morys M., Mims III F.M., Hagerup S., Anderson S.E., A. Baker, J. Kia, T. Wallkup (2001) J.
Geophys. Res., 106, 14573-14582.
1. Holben B.N. et al. (1998), Rem. Sens. Environ., 66, 1-16.
2. Eck T.F., Holben B.N., Reid J.S., Dubovik O., Smirnov A., O’Neill N.T., Slutsker I. and Kinne
S. (1999) J. Geophys. Res., 104, 31333-31350.
3. Smirnov A., Holben B.N., Eck T.F., Dubovik O. and Slutsker I. (2000) Rem. Sens. Env., 73,
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